SCAP Antibody / SREBP cleavage-activating protein

SCAP is a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis.

Product Specifications

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:50-1:100

UniProt

Q12770

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 604-632 from the human protein were used as the immunogen for the SCAP antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity purified

Format

Purified

Limitations

This SCAP antibody is available for research use only.

Storage Conditions

Aliquot the SCAP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SCAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human 1) HeLa, 2) HT-29, 3) K562, 4) LNCaP, 5) mouse brain, 6) mouse liver and 7) rat heart lysate with SCAP antibody. Predicted molecular weight: ~96, 98, 140 kDa (3 isoforms) .