Glutaminase Antibody / GLS

Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981) . High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.

Product Specifications

Specifications

Western blot: 1:1000-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE) : 1:100-1:200, Flow cytometry: 1:25 (per 1x10e6 cells)

UniProt

O94925

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 516-545 from the human protein were used as the immunogen for the Glutaminase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity purified

Format

Purified

Limitations

This Glutaminase antibody is available for research use only.

Storage Conditions

Aliquot the Glutaminase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Glutaminase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Glutaminase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red) .