GCLM Antibody / Glutamate-Cysteine Ligase Regulatory Subunit

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Product Specifications

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:25-50, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

UniProt

P48507

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 246-274 from the human protein were used as the immunogen for the GCLM antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS, IF

Purity

Antigen affinity purified

Format

Purified

Limitations

This GCLM antibody is available for research use only.

Storage Conditions

Aliquot the GCLM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GCLM antibody may be required due to differences in protocols and secondary/substrate sensitivity.