CTLA4 Antibody / Cytotoxic T-lymphocyte protein 4 / CD152

CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.

Product Specifications

Specifications

Western blot: 1:1000-1:2000, Immunofluorescence: 1:50-1:100

UniProt

P16410

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 182-210 from the human protein was used as the immunogen for this CTLA4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Antigen affinity

Format

Antigen affinity purified

Limitations

This CTLA4 antibody is available for research use only.

Storage Conditions

Aliquot the CTLA4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CTLA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) WiDr, 2) Raji, 3) K562, 4) Jurkat and 5) CCRF-CEM cell lysate with CTLA4 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.