Phospho-Histone H2AX Antibody (pS139)
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Product Specifications
Specifications
Western blot: 1:1000-1:2000, Immunofluorescence: 1:200-1:400
UniProt
P16104
Host
Mouse
Reactivity
Human, Mouse
Immunogen
The amino acid sequence surrounding phosphorylated serine 139 was used as the immunogen for the Phospho-Histone H2AX antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Applications
WB, IF
Purity
Antigen affinity purified
Format
Purified
Limitations
This Phospho-Histone H2AX antibody is available for research use only.
Storage Conditions
Aliquot the Phospho-Histone H2AX antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Phospho-Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of mouse NIH 3T3 cells untreated (left) and treated (right) with Hydroxyurea, using Phospho-Histone H2AX antibody.
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