MAPE Antibody / PRAME

MAPE (Melanoma antigen preferentially expressed in tumors), also called PRAME (Preferentially expressed antigen of melanoma), is a protein that is preferentially expressed in tumors, particularly in melanoma. It plays a vital role in regulating cell proliferation, migration, and invasion, making it a promising target for therapeutic interventions. Studies have shown that the overexpression of MAPE protein is associated with aggressive melanoma subtypes, indicating its potential as a prognostic biomarker in melanoma patients.

Product Specifications

Specifications

Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:25 per million cells in 0.1ml

UniProt

P78395

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 476-502 from the human protein was used as the immunogen for the MAPE antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity

Format

Purified

Limitations

This MAPE antibody is available for research use only.

Storage Conditions

Aliquot the MAPE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MAPE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasm, Nucleus, Cell Membrane

Image Legend

IHC staining of FFPE human testis tissue with MAPE antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.