MT-ND3 Antibody / NADH-ubiquinone oxidoreductase chain 3

MT-ND3 is a critical subunit of complex I, acting as a catalyst in the transfer of electrons from NADH to ubiquinone. This process is essential for generating the proton gradient necessary for ATP production. Without MT-ND3, the electron transport chain would not be able to function properly, leading to a decrease in ATP production and ultimately, cellular dysfunction. Research has shown that mutations in the gene encoding MT-ND3 can lead to mitochondrial diseases and a range of health problems, including muscle weakness, neurological disorders, and even premature aging.

Product Specifications

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 per million cells in 0.1ml

UniProt

P03897

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 10-38 from the human protein was used as the immunogen for this MT-ND3 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS

Purity

Antigen affinity

Format

Antigen affinity purified

Limitations

This MT-ND3 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the MT-ND3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MT-ND3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.