Histone H3 (acetyl K14) Antibody / HIST1H3A

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate

UniProt

P68431

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Histone H3 (acetyl K14) was used as the immunogen for the Histone H3 (acetyl K14) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

BID-8

Applications

WB, IHC, ICC, IF, IP

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Histone H3 (acetyl K14) antibody is available for research use only.

Storage Conditions

Store the Histone H3 (acetyl K14) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Histone H3 (acetyl K14) antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC analysis of Histone H3 (acetyl K14) using anti-Histone H3 (acetyl K14) antibody. Histone H3 (acetyl K14) was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Histone H3 (acetyl K14) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.