ERC1 Antibody / ELKS/RAB6-interacting/CAST family member 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q8IUD2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human ERC1 recombinant protein (Position: H721-S1116) was used as the immunogen for the ERC1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC/IF, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This ERC1 antibody is available for research use only.

Storage Conditions

After reconstitution, the ERC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the ERC1 antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunoprecipitating ERC1 in Hela whole cell lysate. Western blot analysis of ERC1 using anti-ERC1 antibody. Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ERC1 antibody in Hela whole cell lysate, Lane 3: anti-ERC1 antibody (2ug) + Hela whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERC1 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected band size for ERC1 is at 128 kDa.