Phospho-Src (Tyr529) Antibody

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

P12931

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-Src (Y529) was used as the immunogen for the Phospho-Src (Tyr529) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32S01

Applications

WB, ICC, IF

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-Src (Tyr529) antibody is available for research use only.

Storage Conditions

Store the Phospho-Src (Tyr529) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-Src (Tyr529) antibody should be determined by the researcher.

Image Legend

All lanes use the antibody at 1:3K dilution for 1 hour at room temperature. Expected size for c-Src (phospho-Tyr529) ~60 kDa. Observed (possible) doublet at ~55 kDa and ~60 kDa likely reflects full-length and slightly modified/truncated phosphorylated forms of c-Src, consistent with literature indicating Src family kinases migrate in the 55-62 kDa range.