UBE4B Antibody / Ubiquitin conjugation factor E4 B
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
O95155
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human UBE4B recombinant protein (Position: S184-H1302) was used as the immunogen for the UBE4B antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ICC/IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This UBE4B antibody is available for research use only.
Storage Conditions
After reconstitution, the UBE4B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the UBE4B antibody should be determined by the researcher.
Image Legend
IF analysis of UBE4B using anti-UBE4B antibody (green) . UBE4B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-UBE4B antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS1047463-01 0.02 mg (E-Coli)
Recombinant Bacillus amyloliquefaciens Elongation factor P (efp)
Sign In for Pricing
View Details