HNRNPM Antibody / heterogeneous nuclear ribonucleoprotein M

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Flow Cytometry: 1:50

UniProt

P52272

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human HNRNPM was used as the immunogen for the HNRNPM antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

29H77

Applications

WB, IHC, ICC, IF, FACS

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This HNRNPM antibody is available for research use only.

Storage Conditions

Store the HNRNPM antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the HNRNPM antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC analysis of HNRNPM using anti-HNRNPM antibody. HNRNPM was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HNRNPM antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.