Phospho-Tau (Ser404) Antibody / MAPT

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50

UniProt

P10636

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-Tau (S404) was used as the immunogen for the Phospho-Tau (Ser404) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31M20

Applications

WB, IHC, ICC, IF, IP

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-Tau (Ser404) antibody is available for research use only.

Storage Conditions

Store the Phospho-Tau (Ser404) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-Tau (Ser404) antibody should be determined by the researcher.

Image Legend

All lanes use the Phospho-Tau (Ser404) antibody at 1:2K dilution for 1 hour at room temperature. The expected molecular weight of phosphorylated Tau (Ser198) is approximately 45-70 kDa, corresponding to the major Tau isoforms, and lower molecular weight bands around ~35 kDa may represent truncated Tau fragments commonly observed in brain tissue.