UBE2T Antibody / Ubiquitin-conjugating enzyme E2 T / HSPC150

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q9NPD8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human UBE2T recombinant protein (Position: R9-Q145) was used as the immunogen for the UBE2T antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This UBE2T antibody is available for research use only.

Storage Conditions

After reconstitution, the UBE2T antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the UBE2T antibody should be determined by the researcher.

Image Legend

IF analysis of UBE2T using anti-UBE2T antibody (green) . UBE2T was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:100 rabbit anti-UBE2T antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.