Phospho-Tau (Ser214) Antibody / MAPT

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

UniProt

P10636

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human Phospho-Tau (S214) was used as the immunogen for the Phospho-Tau (Ser214) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31M21

Applications

WB, IHC

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-Tau (Ser214) antibody is available for research use only.

Storage Conditions

Store the Phospho-Tau (Ser214) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-Tau (Ser214) antibody should be determined by the researcher.

Image Legend

All lanes use the Phospho-Tau (Ser214) antibody at 1:1k dilution for 1 hour at room temperature. The expected molecular weight of phosphorylated Tau (Ser198) is approximately 45-70 kDa, corresponding to the major Tau isoforms, and lower molecular weight bands around ~35 kDa may represent truncated Tau fragments commonly observed in brain tissue.