UBE2A/UBE2B Antibody / Ubiquitin-conjugating enzyme E2
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P49459|P63146
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human UBE2A/2B recombinant protein (Position: M1-C152) was used as the immunogen for the UBE2A/UBE2B antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This UBE2A/UBE2B antibody is available for research use only.
Storage Conditions
After reconstitution, the UBE2A/UBE2B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the UBE2A/UBE2B antibody should be determined by the researcher.
Image Legend
IHC analysis of UBE2A/UBE2B using anti-UBE2A/UBE2B antibody. UBE2A/UBE2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-UBE2A/UBE2B antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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