Syncoilin Antibody / Sync

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9EPM5

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

E.coli-derived mouse Syncoilin/Sync recombinant protein (Position: D11-Q465) was used as the immunogen for the Syncoilin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This Syncoilin antibody is available for research use only.

Storage Conditions

After reconstitution, the Syncoilin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the Syncoilin antibody should be determined by the researcher.

Image Legend

Western blot analysis of SYNC using anti-SYNC antibody. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat H9C2 (2-1) whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNC antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for SYNC at approximately 72 kDa. The expected band size for SYNC is at 54 kDa but is also observed at 65-70 kDa.