ARHGEF12 Antibody / Rho guanine nucleotide exchange factor 12

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

Q9NZN5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: D10-E1426) was used as the immunogen for the ARHGEF12 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IP, IF, IHC, ICC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This ARHGEF12 antibody is available for research use only.

Storage Conditions

After reconstitution, the ARHGEF12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the ARHGEF12 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunohistochemical staining of ARHGEF12L using anti-ARHGEF12L antibody. ARHGEF12L was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-ARHGEF12L antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.