SRRT Antibody / ARS2
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q9BXP5
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human ARS2/SRRT recombinant protein (Position: H125-Q793) was used as the immunogen for the SRRT antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC, IF, IP, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This SRRT antibody is available for research use only.
Storage Conditions
After reconstitution, the SRRT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SRRT antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunohistochemical staining of SRRT using anti-SRRT antibody. SRRT was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SRRT antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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