LMO2 Antibody / Rhombotin 2

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

P25791

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human LMO2 recombinant protein (Position: A4-E150) was used as the immunogen for the LMO2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This LMO2 antibody is available for research use only.

Storage Conditions

After reconstitution, the LMO2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the LMO2 antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of K562 cells using anti-LMO2 antibody. Overlay histogram showing K562 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LMO2 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.