Ifng Antibody / Ifn gamma
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL
UniProt
P01580
Host
Rabbit
Reactivity
Mouse
Immunogen
E.coli-derived mouse IFN Gamma/Ifng recombinant protein (Position: H23-C155) was used as the immunogen for the Ifng antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This Ifng antibody is available for research use only.
Storage Conditions
After reconstitution, the Ifng antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the Ifng antibody should be determined by the researcher.
Image Legend
Western blot analysis of IFN Gamma/Ifng using anti-Ifng antibody. Lane 1: recombinant mouse Ifng protein 5 ng, Lane 1: recombinant mouse Ifng protein 2.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ifng antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent.
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