GBP1 Antibody / Guanylate-binding protein 1
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
P32455
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human GBP1 recombinant protein (Position: E190-S592) was used as the immunogen for the GBP1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, FACS, IP, IF, IHC, ICC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This GBP1 antibody is available for research use only.
Storage Conditions
After reconstitution, the GBP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the GBP1 antibody should be determined by the researcher.
Location
Cytoplasmic, secreted
Image Legend
Immunohistochemical staining of GBP1 using anti-GBP1 antibody. GBP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-GBP1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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