CD21 Antibody / Complement receptor type 2
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL
UniProt
P20023
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
E.coli-derived human CD21/CR2 recombinant protein (Position: D76-N817) was used as the immunogen for the CD21 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This CD21 antibody is available for research use only.
Storage Conditions
After reconstitution, the CD21 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Product Datasheet
http://www.nsjbio.com/tds-pdf/cd21-antibody-complement-receptor-type-2-cr2-fy12216-nsj-bioreagents
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the CD21 antibody should be determined by the researcher.
Image Legend
Western blot analysis of CD21/CR2 using anti-CD21 antibody. Lane 1: mouse Raw264.7 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD21 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. The expected band size for CD21/CR2 is at 110-120 kDa (multiple isoforms), but it may be observed at higher molecular weights due to glycosylation.
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