Phospho-MAP2 (Ser136) Antibody / Microtubule-associated protein 2

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

P11137

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-MAP2 (S136) was used as the immunogen for the Phospho-MAP2 (Ser136) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32M37

Applications

WB, IHC, ICC, IF

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-MAP2 (Ser136) antibody is available for research use only.

Storage Conditions

Store the Phospho-MAP2 (Ser136) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-MAP2 (Ser136) antibody should be determined by the researcher.

Image Legend

All lanes use the Phospho-MAP2 (Ser136) antibody at 1:1K dilution for 1 hour at room temperature. Phospho-MAP2 (Ser136) signal was detected only in brain lysates, consistent with MAP2 being a neuron-specific protein that is naturally phosphorylated in neural tissue and absent from non-neural organs such as liver, spleen, lung, kidney, and skin.