PLS3 Antibody / Plastin 3

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

P13797

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human PLS3 recombinant protein (Position: M1-D580) was used as the immunogen for the PLS3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IP, IF, ICC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This PLS3 antibody is available for research use only.

Storage Conditions

After reconstitution, the PLS3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the PLS3 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of PLS3 using anti-PLS3 antibody (green) . PLS3 was detected in an immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PLS3 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.