AXIN1 Antibody / Axin-1

After reconstitution, the AXIN1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of AXIN1 using anti-AXIN1 antibody. Lane 1: human whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXIN1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. AXIN1 antibody detects one or two bands between 90-100 kDa in the indicated cell lysates, consistent with full-length AXIN1 and its phosphorylated form. A smaller band at ~70 kDa is observed in A549 and K562 lysates, likely representing a truncated C-terminal fragment generated by regulated proteolysis or caspase cleavage.