NASP antibody / Nuclear autoantigenic sperm
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
P49321
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human NASP recombinant protein (Position: K442-C788)
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC, IF, IP, FACS, ELISA
Purity
Immunogen affinity purified
Format
Liquid
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Limitations
This NASP antibody is available for research use only.
Storage Conditions
Store the NASP antibody at -20°C.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the NASP antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of NASP using anti-NASP antibody (red) . NASP was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-NASP antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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