NRTN Antibody / Neurturin
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
Q99748
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human Neurturin/NRTN recombinant protein (Position: M1-V197) was used as the immunogen for the NRTN antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IHC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Limitations
This NRTN antibody is available for research use only.
Storage Conditions
After reconstitution, the NRTN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the NRTN antibody should be determined by the researcher.
Image Legend
Immunohistochemical staining of NRTN using anti-NRTN antibody. NRTN was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NRTN antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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