NRTN Antibody / Neurturin

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

Q99748

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human Neurturin/NRTN recombinant protein (Position: M1-V197) was used as the immunogen for the NRTN antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Limitations

This NRTN antibody is available for research use only.

Storage Conditions

After reconstitution, the NRTN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the NRTN antibody should be determined by the researcher.

Image Legend

Immunohistochemical staining of NRTN using anti-NRTN antibody. NRTN was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NRTN antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.