Histone H3 (mono methyl K36) Antibody / HIST1H3A

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

P68431

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Histone H3 (mono methyl K36) was used as the immunogen for the Histone H3 (mono methyl K36) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

DEG-8

Applications

WB, ICC, IF

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Histone H3 (mono methyl K36) antibody is available for research use only.

Storage Conditions

Store the Histone H3 (mono methyl K36) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Histone H3 (mono methyl K36) antibody should be determined by the researcher.

Image Legend

Western blot analysis of Histone H3 using Histone H3 (mono methyl K36) antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antibody at 1:1000 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for Histone H3 at approximately 15-17 kDa. The expected molecular weight of Histone H3 is at 15 kDa.