ARID3A Antibody / AT-rich interactive domain-containing protein 3A

Product Specifications

Specifications

Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Western Blot: 0.25-0.5 µg/mL

UniProt

Q99856

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ARID3A was used as the immunogen for the ARID3A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IP, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This ARID3A antibody is available for research use only.

Storage Conditions

After reconstitution, the ARID3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the ARID3A antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of Caco-2 cells using anti-ARID3A antibody. Overlay histogram showing Caco-2 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID3A antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.