NCF1 Antibody / Neutrophil cytosolic factor 1 / p47phox
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
P14598
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human p47 phox/NCF1 recombinant protein (Position: Q22-R384) was used as the immunogen for the NCF1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, FACS, IF, IHC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This NCF1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NCF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the NCF1 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunohistochemical staining of NCF1 using anti- NCF1 antibody. NCF1 was detected in a paraffin-embedded section of rat lymph tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti- NCF1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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