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OCLN Antibody / Occludin

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

UniProt

Q16625

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human Occludin/OCLN recombinant protein (Position: S358-Q520) was used as the immunogen for the OCLN antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, IP, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This OCLN antibody is available for research use only.

Storage Conditions

After reconstitution, the OCLN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

http://www.nsjbio.com/tds-pdf/ocln-antibody-occludin-fy12345-nsj-bioreagents

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the OCLN antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

Immunohistochemical staining of OCLN using anti-OCLN antibody. OCLN was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-OCLN antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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