SEC61B Antibody / Sec61 subunit beta

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Western Blot: 0.25-0.5 µg/mL

UniProt

P60468

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human SEC61B recombinant protein (Position: R16-S96) was used as the immunogen for the SEC61B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SEC61B antibody is available for research use only.

Storage Conditions

After reconstitution, the SEC61B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SEC61B antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of PC-3 cells using anti-SEC61B antibody. Overlay histogram showing PC-3 cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC61B antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.