PTEN Antibody / Phosphatase and tensin

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL

UniProt

P60484

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human PTEN was used as the immunogen for the PTEN antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This PTEN antibody is available for research use only.

Storage Conditions

After reconstitution, the PTEN antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the PTEN antibody should be determined by the researcher.

Image Legend

Western blot analysis of PTEN using anti-PTEN antibody. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTEN antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Expected molecular weight: 47~55 kDa (isoform 1), 65~70 kDa (' long' isoform) .