RSPO4 Antibody / R-spondin-4

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q2I0M5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human RSPO4 recombinant protein (Position: R60-K206) was used as the immunogen for the RSPO4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This RSPO4 antibody is available for research use only.

Storage Conditions

After reconstitution, the RSPO4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the RSPO4 antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of 293T cells using anti-RSPO4 antibody. Overlay histogram showing 293T cells stained with (Blue line) . The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RSPO4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.