ELF3 Antibody / E74-like factor 3

After reconstitution, the ELF3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunoprecipitation of ELF3 in whole cell lysate. Western blot analysis of ELF3 using anti-ELF3 antibody. Lane 1: whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ELF3 antibody in whole cell lysate, Lane 3: anti-ELF3 antibody (2ug) + whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELF3 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. ELF3 (~41 kDa predicted) was detected as bands at ~50 kDa, and ~100 kDa. The ~50 kDa form corresponds to phosphorylated/SUMO-modified ELF3, while the ~100 kDa species likely represents a dimeric or complex-associated form, consistent with published reports.