Spink5 Antibody / Serine protease inhibitor Kazal-type 5

After reconstitution, the Spink5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of Spink5 using anti-Spink5 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: rat stomach tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK5 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. Spink5 (~121 kDa predicted) was detected at ~105 kDa, consistent with the partially processed, glycosylated precursor described in keratinocyte and epithelial studies.