BBS1 Antibody / Bardet-Biedl syndrome 1

After reconstitution, the BBS1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of BBS1 using anti-BBS1 antibody. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBS1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. BBS1 (~65 kDa predicted) was detected as bands at ~65 kDa and ~55 kDa. The higher band corresponds to the full-length, post-translationally modified BBSome-associated form, while the lower band likely represents a dephosphorylated or partially processed variant, consistent with published reports.