HSPA6 Antibody / Heat shock 70 kDa protein 6

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P17066

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HSPA6 was used as the immunogen for the HSPA6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This HSPA6 antibody is available for research use only.

Storage Conditions

After reconstitution, the HSPA6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the HSPA6 antibody should be determined by the researcher.

Image Legend

Western blot analysis of HSPA6 using anti-HSPA6 antibody. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA6 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The expected molecular weight of HSPA6 is ~71 kDa.