PGM1 Antibody / Phosphoglucomutase 1

After reconstitution, the PGM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of PGM1 using anti-PGM1 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat skin tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skin tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. PGM1 (~61 kDa predicted) was detected primarily at ~70 kDa, consistent with phosphorylation-dependent migration shifts. A secondary ~40 kDa band in human samples corresponds to a known N-terminal proteolytic fragment reported in mammalian cells.