POMGNT2 Antibody / Protein O-linked mannose N-acetylglucosaminyltransferase 2

After reconstitution, the POMGNT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of POMGNT2 using anti-POMGNT2 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMGNT2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. POMGNT2 (~67 kDa predicted) was detected primarily at ~75–90 kDa, consistent with N-linked glycosylation that slows migration on SDS-PAGE. Additional higher and lower bands in rodent tissues likely represent differentially glycosylated or partially processed enzyme forms.