MARCKS Antibody / Myristoylated alanine-rich C-kinase substrate
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P29966
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MARCKS was used as the immunogen for the MARCKS antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, FACS
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This MARCKS antibody is available for research use only.
Storage Conditions
After reconstitution, the MARCKS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the MARCKS antibody should be determined by the researcher.
Location
Cytoplasm, cell membrane
Image Legend
Immunohistochemical staining of MARCKS using anti-MARCKS antibody. MARCKS was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MARCKS antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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