MCMBP Antibody / MCM-binding protein

After reconstitution, the MCMBP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunoprecipitation of MCMBP in 293T whole cell lysate. Western blot analysis of MCMBP using anti-MCMBP antibody. Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-MCMBP antibody in 293T whole cell lysate, Lane 3: anti-MCMBP antibody (2ug) + 293T whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCMBP antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected molecular weight of MCMBP is ~73 kDa.