MCMBP Antibody / MCM-binding protein

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

UniProt

Q9BTE3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human MCMBP recombinant protein (Position: E234-E534) was used as the immunogen for the MCMBP antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IP, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This MCMBP antibody is available for research use only.

Storage Conditions

After reconstitution, the MCMBP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the MCMBP antibody should be determined by the researcher.

Image Legend

Immunoprecipitation of MCMBP in 293T whole cell lysate. Western blot analysis of MCMBP using anti-MCMBP antibody. Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-MCMBP antibody in 293T whole cell lysate, Lane 3: anti-MCMBP antibody (2ug) + 293T whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCMBP antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. The expected molecular weight of MCMBP is ~73 kDa.