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SLC7A11 Antibody / XCT / Solute carrier family 7 member 11

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q9UPY5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A peptide specific to the SLC7A11 protein was used as the immunogen for the SLC7A11 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SLC7A11 antibody is available for research use only.

Storage Conditions

After reconstitution, the SLC7A11 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SLC7A11 antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

Immunohistochemical staining of xCT/SLC7A11 using anti-SLC7A11 antibody. xCT/SLC7A11 was detected in a paraffin-embedded section of human ovraian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SLC7A11 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.

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