PAN3 Antibody / PAN deadenylation complex subunit 3
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q58A45
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
E.coli-derived human PAN3 recombinant protein (Position: Q227-L887) was used as the immunogen for the PAN3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC/IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This PAN3 antibody is available for research use only.
Storage Conditions
After reconstitution, the PAN3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the PAN3 antibody should be determined by the researcher.
Location
Cytoplasmic, Nuclear
Image Legend
Immunohistochemical staining of PAN3 using anti-PAN3 antibody. PAN3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAN3 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
