SNCB Antibody / Beta Synuclein

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q91ZZ3

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SNCB was used as the immunogen for the SNCB antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, IF

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SNCB antibody is available for research use only.

Storage Conditions

After reconstitution, the SNCB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SNCB antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunohistochemical staining of SNCB using anti-SNCB antibody. SNCB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SNCB antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.