STK17A Antibody / Serine/threonine-protein kinase 17A

After reconstitution, the STK17A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunoprecipitating STK17A in U251 whole cell lysate. Western blot analysis of STK17A using anti-STK17A antibody. Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-STK17A antibody in U251 whole cell lysate, Lane 3: anti-STK17A antibody (2ug) + U251 whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STK17A antibody at a dilution of 0.5 ug/ml and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain) . The signal is developed using ECL Plus Western Blotting Substrate. STK17A (~47 kDa predicted) was detected as a major band at ~53 kDa, consistent with phosphorylation-dependent migration shift and the acidic C-terminal domain known to cause slower SDS-PAGE mobility.