STK17A Antibody / Serine/threonine-protein kinase 17A

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q9UEE5

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human STK17A recombinant protein (Position: Q38-Q383) was used as the immunogen for the STK17A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This STK17A antibody is available for research use only.

Storage Conditions

After reconstitution, the STK17A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the STK17A antibody should be determined by the researcher.

Image Legend

Immunoprecipitating STK17A in U251 whole cell lysate. Western blot analysis of STK17A using anti-STK17A antibody. Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-STK17A antibody in U251 whole cell lysate, Lane 3: anti-STK17A antibody (2ug) + U251 whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STK17A antibody at a dilution of 0.5 ug/ml and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain) . The signal is developed using ECL Plus Western Blotting Substrate. STK17A (~47 kDa predicted) was detected as a major band at ~53 kDa, consistent with phosphorylation-dependent migration shift and the acidic C-terminal domain known to cause slower SDS-PAGE mobility.