KIF7 Antibody / Kinesin-like protein KIF7
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL
UniProt
Q2M1P5
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human KIF7 was used as the immunogen for the KIF7 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This KIF7 antibody is available for research use only.
Storage Conditions
After reconstitution, the KIF7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the KIF7 antibody should be determined by the researcher.
Image Legend
Western blot analysis of KIF7 using anti-KIF7 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF7 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for KIF7 at approximately 151 kDa. The expected molecular weight of KIF7 is ~151 kDa.
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